Hi Lucas, Yes, here is one thing you can do: (#1 will be by hand for the version that you have unfortunately): 1. Use the MR solution to calculate phases; get an anomalous difference Fourier, and pick the top peaks as your heavy-atom sites. 2. Read in the anomalous data and sites to AutoSol and get experimental phases. 3. Read in the experimental phases and the MR model to AutoBuild, with rebuild_in_place=False (build model from scratch) and include_input_model=True (default) and let it try to density modify and continue on with model rebuilding starting from the 25% model. Good luck! -Tom T At 08:13 AM 6/16/2007, Lucas Bleicher wrote:
I have a dataset from a Cs-soaked crystal, and the protein has a domain solved in the PDB. The problem is that the map obtained just with the experimental phasing is not so easily interpreted, and the domain is just about 25% of the whole protein. Inspection of the map generated by MR suggests that it's a good solution, with residues fitting reasonably well on the density.
Given the new features on Phaser 2.0, which I suppose is included in Phenix 1.3b, can anyone suggest a protocol to profit from both things (anomalous scattering + good search model)?
Thanks in advance, Lucas
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