Hi everyone,
I have came into a problem recently. My structure was solved by MAD and a crude model was built manually.
Several peak data sets were collected, resolution is about 4A-4.2A. The heavy atom can be located by anomalous difference fourier. I feed the initial phase or heavy atom sites, and the peak data set to phenix. However the map after DM is too bad. The density is very strange. I don't know what's the problem?
In addition, how to define protein mask at phenix autosol for density modification?
Thanks in advance.