Re: [phenixbb] ramachanran plot refinement or restraint?

Jianghai Zhu wrote:

Adding riding hydrogens into the structure was a powerful validation tool. But now we all add riding hydrogens into the refinement since it makes the refinement better. At the same time, we lose some power in the validation. We still think it is worth doing so, don't we?

No: they are RIDING hydrogens, they do not influence the refinement (i.e. bumps), only the Rfactors/maps. Which is not the same as you ask for with the phi/psi angles. In general, if your model is correct (and the error can be a few residues away), the backbone won't move around, even at low resolution. Cheers phx