Thanks for the reply Pavel However, with regard to the NCS maps issue, which should I be building to?Does the 2fofoc that phenix puts out adjusted for ncs and use that? Thanks FR On Aug 25, 2008, at 11:59 AM, Pavel Afonine wrote:
Hi Francis,
Also if anyone has a paper/protocol for NCS refinement, I'd love to check it out (to figure out which map to fit to).
Just something to keep in mind... I always prefer to run two refinements in parallel with and without using NCS. It's resolution (and data-to-parameters ratio) dependent. I've seen a number of cases where using the NCS is very beneficial and similar number of cases where it's better to not use NCS (mostly at resolutions ~2-2.5A).
I would also check manually the NCS groups that are found by phenix.refine automatically. I have some cases where automatic detection did not yield a good selection.
Pavel.
_______________________________________________ phenixbb mailing list [email protected] http://www.phenix-online.org/mailman/listinfo/phenixbb
--------------------------------------------- Francis Reyes M.Sc. 215 UCB University of Colorado at Boulder gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D 8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D