Raja Dey wrote:
Dear Friends,
Does anyone have experience of building dna in coot? I can see the clear density, but I need to move some nucleotides. Any suggestion/direction is welcome.
Dear Raja, (Not that I've done a lot of this you understand) but what you need to do depends on how bad your model is (and how good your map is). If it's close you can simply click and drag around a residue range (more than 3 residues takes some experience to handle correctly (generally I don't do that)). If the model is a long way off, I'd delete the bits that don't fit to density - and build off a well-fitting stump using add terminal residue and simple mutate (and then refine). And repeat. You might find the Watson-Crick Base Pair tool useful. HTH, Paul.