On Tue, 2011-03-15 at 11:45 -0400, Zhang yu wrote:
Fast Rotation Function Table: 1 ------------------------------- #SET Top (Z) Second (Z) Third (Z) 1 -1129.09 32.53 --- --- --- --- ---- ---------- ----- ---------- ----- ---------- -----
Well, your Z-score is very promising. What size protein/DNA you have? The likely asu content is over a megadalton, so unless the protein is huge, you probably have more than one copy - this would explain bad initial R-values. Patterson suggests that too. You probably need to play with the estimate of asu content a bit to get rid of negative LLG. What happens if you run a search looking for the second copy with the first one in place? -- "I'd jump in myself, if I weren't so good at whistling." Julian, King of Lemurs