Hello, Definitely try microseeding: take a big crystal, put it in eppendorf with mother liquor ( the precipitant should be at a concentration higher than what you use to build the big crystals) and crash it by vortexing (put a small piece of plastic in the tube). If you use the vapor diffusion technique take a µl of this solution and mix it with your protein solution on a cover slip just as if you set a normal drop, and then seal the well. Normally youll get a lot of tiny crystals after day or two. Good luck Filip On 2012-07-05 01:55, jens j birktoft wrote:
Hi everybody,
Any suggestions regarding how to generate small!! crystals? Yes, in this case that is what we want While we can generate crystals of size 100-500 microns, this time we something in the range of .5-5 microns. Any ideas and suggestions would be greatly appreciated,
Cheers
Jens