Dear colleagues,

 

Please prompt me on correctly applying the symmetry parameters to the helical cryo-em map.

 

What I did:

  1. Found symmetry with Map symmetry. The process finished successfully.
  2. Applied the NFC operators.  

But the outcome has only two units (C2 symmetry).
When I put more units in the program setting, nothing happens after the run; moreover, the output is not generated.

 

 Could you please advise how to fill the helical cryo-EM structure with units?

 
Many thanks for considering my request.

 

Sincerely,

Dmitry