9 Jul
2018
9 Jul
'18
6:32 p.m.
Hi Kaibo,
2. The 2Fo-Fc map and AutoBuild solution looked fine, but the Fo-Fc map is kinda noisy with positive peaks showing up within the main-chain density which causes troubles for refinement.
what contouring level you use to see Fo-Fc map? Note, usual "3 sigma" is not universally optimal but depends on solvent content. Do you still see lots of artifacts at say 0.35 or 0.4e/A**3 (mean solvent density) ? Pavel