I know a partial to this question. You want to exclude some residues from the ncs refinement. In the .def file give something like this: restraint_group { reference = "chain F and (resid 17:300 and not (resid 176 or resid 179 or resid 238 or resid 242) )" selection = "chain E and (resid 17:300 and not (resid 176 or resid 179 or resid 238 or resid 242) )" selection = "chain D and (resid 17:300 and not (resid 176 or resid 179 or resid 238 or resid 242) )" coordinate_sigma = 0.05 b_factor_weight = 10 } Mirek Cygler wrote:
Hi, I have NCS symmetry in my crystal and wouldlike to use it during the refinement. What is the strength of the restraints for the backbone, for the sidechains? How can I control these restraints? Thanks,
Mirek
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