Fred
If you change the input geometry to be C3'-endo, phenix.refine will
maintain the geometry by applying the appropriate restraints. I can see how
specifying the desired pucker would be nice. If you send me the files, I
will work on it.
Cheers
Nigel
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Nigel W. Moriarty
Building 33R0349, Molecular Biophysics and Integrated Bioimaging
Lawrence Berkeley National Laboratory
Berkeley, CA 94720-8235
Phone : 510-486-5709 Email : [email protected]
Fax : 510-486-5909 Web : CCI.LBL.gov
On Fri, Apr 27, 2018 at 1:54 PM, Dyda
Hi,
Is it possible and if so how to change the pucker restraint for a particular DNA nucleotide from C2'-endo to C3'-endo in phenix.refine? I have a problem with a terminal nucleotide that is in an active site and the current C2'-endo doesn't seem consistent with the 2Fo-Fc map.
Thanks for your help.
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