On Sep 16, 2009, at 11:51 AM, Pei-Chun Lin wrote:
I have spent several months working on a MAD data (3.5 Ang) and already build 70% of the model (R/Rfree=30/40). Recently, I collected two dataset (pk and infl, both 3.1 Ang) from single point mutation, and native data(3.3 Ang). I first tried to use phenix autosol to generate a new map by using point mutation dataset, but failed to generate a reasonable map for unknown reason (phenix complained different cell dimensions)
If the cell dimensions are significantly different, rebuilding the model using both the 3.5A and 3.1A datasets will probably not work. The model is definitely good enough to try running molecular replacement with the 3.1A data (either wavelength - whichever is higher-quality). If you use AutoMR it will also automatically run AutoBuild. (As for the hires_file, you can only specify one of these.) ------------------- Nathaniel Echols Lawrence Berkeley Lab 510-486-5136 [email protected]