Hi Matteo, you are right, the absolute value contains the contribution from all absorbing elements. The edge can be determined reliably, though, because it is not shifted with respect to the energy (by this effect), only with respect to the amplitude. Best, Tim On 10/20/2014 02:44 PM, Matteo Colombo wrote:
Hello, I was wondering if the f'' determined by XANES experiment is quantitatively significant and if it can be contributed by two different anomalous scatterers.
For example in my protein I know that Co and Zn are present. In theory, at Zn absorption edge, I have a contribution from Co too. If I perform a XANES experiment to determine Zn absorption edge and I obtain a f'' of 5.87, does this value correspond to only Zn signal or to a sum of Zn+Co?
Thanks a lot
Matteo
-- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A