Dear All,
After we refine the structure of the protein to satisfactory with satisfactory Rwork and Rfree, we pick water by phenix refine, and I find Rfree always increases slightly after the water picking refinment.
Do you have nay idea to solve this problem or any comment?
Cheers,
Dialing
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From: Nathaniel Echols
But how about if both the Rfree and Rwork decrease, by comparing the start and final, however in the middle of the the refinement (for example the second cycle of the default3 cycles) the Rfree and Rwork are higher in comparison with the Rfree_start and Rwork_statrt?
This is okay, and not at all uncommon (especially if you use simulated annealing). -Nat _______________________________________________ phenixbb mailing list [email protected] http://phenix-online.org/mailman/listinfo/phenixbb