[phenixbb] Re: cryo em map

Hi all, Can you please advise once we fit the map and open in coot. What is next step to do in coot. I tried refmac but that required ccp4 file which doesn't have. Please some one can help in elaborating and sharing any tutorial would be helpful. I'm new bie and first time I am trying model building. Any help would be really appreciated. Thank you so much Best wishes Tim On Tue, 17 Dec 2024, 13:47 Oleg Sobolev, wrote:

Hello Iram,

The general workflow for Cryo-EM is described here: https://phenix-online.org/documentation/overviews/cryo-em_index.html

It looks like you are ready to refine your model, so the tool for it is phenix.real_space_refine: https://phenix-online.org/documentation/reference/real_space_refine.html that also has a video tutorial.

Let us know if you have any questions.

Best regards, Oleg Sobolev.

On Thu, Dec 12, 2024 at 5:54 PM iram aziz wrote:

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Hello All. I got a nice 2.45 Angstrom map from cryo em data. I checked handedness, map model fitting on the chimera and opened in the coot. It looks nice. Can you please give me suggestions about the Phenix refinement steps. Thanks Iram _______________________________________________ phenixbb mailing list -- [email protected] To unsubscribe send an email to [email protected] Unsubscribe: phenixbb-leave@%(host_name)s

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