On Thu, Aug 5, 2010 at 7:53 AM, Hermella Woldemdihin
Where can I set B-factor for water in phenix.refine on the GUI version of phenix?
Do you mean resetting the B-factors for all waters, or the B-factor for new solvent molecules when the water picking option is selected? For the latter, it is here: Settings menu->Ordered solvent...->Initial B-factor value (You may need to change the "user level" menu to "intermediate" or "advanced" if you don't see that control at first.) If you want to reset B-factors for existing waters, open Settings menu->Model modifications->Modify ADPs, enter the selection for waters in the first control ("resname HOH" is usually all you need), and enter a suitable value in "Set isotropic B to:". Of course in either case, by default phenix.refine will re-refine those initial values. -Nat