Re: [phenixbb] Phasing using CryoEM Map

Hi Alex,

I trying to phase my 4Å data with low resolution cryoEM maps (various 12-18Å). There is some documentation, but not really for dealing with EM data. I am having trouble converting the EM data from mrc to suitable F's for phaser.

isn't it a matter of Fourier transforming your map into structure factors and then using them down the road pretending that these are your "Fobs" and however obtained phases? If that's the case then phenix.map_to_structure_factors is the tool you need: http://www.phenix-online.org/documentation/reference/map_to_structure_factor... If not I wonder what am I missing? Pavel