- phenixbb - phenix-online.org

on phenix uninstall
by Smith Lee 13 Feb '21

13 Feb '21

Dear All, In order to install new version of phenix, should I uninstall the older version of phenix first? If needed, what are the command to uninstall the windows version of phenix and to uninstall the linux version of phenix? Smith

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The weblinks for the Daresbury Laue software source code and information
by John R Helliwell 12 Feb '21

12 Feb '21

Dear Colleagues, The weblinks for the Daresbury Laue software source code and information have become hidden from a simple google search. This short article at IUCr Journals makes clear where the software source code and information can now be found. See:- https://journals.iucr.org/s/issues/2021/02/00/me6120/index.html Best wishes, John Emeritus Professor John R Helliwell DSc

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unable to run coot
by Badmalia, Maulik 12 Feb '21

12 Feb '21

hello team phenix, I hope this email finds you well. I recently downloaded phenix for analysing our cryoEM data, before moving to the data analysis I decided to try the tutorial. I decided to use the autosharpen option first, I was able to access the tutorial but when I clicked on coot I repeatedly got a message saying that "Sorry: the 'coot' command could not be located. We have installed Coot to run on a CentOS platform and has the latest version of Coot on the system. I am a novice with linux and phenix. So any help in this regard would be highly appreciated. Do let me know if you have any questions. Regards, Maulik Maulik D. Badmalia Postdoctoral Researcher Laboratory of Medicinal Biophysics Alberta RNA Research and Training Institute Dept. of Chemistry & Biochemistry University of Lethbridge, Lethbridge, AB, Canada

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Re: [phenixbb] unable to run coot
by Werther, Rachel A 12 Feb '21

12 Feb '21

Hello Maulik, Sometimes uninstalling Xquartz and reinstalling it will correct the link between Phenix and Coot, particularly after computer updates. Best of luck, Rachel Rachel Werther / she & her pronouns / Research Technician III / Campbell Lab / Lab A3-201 / 206-667-4189 / Basic Sciences Division / Fred Hutchinson Cancer Research Center / rwerther(a)fredhutch.org<mailto:[email protected]> From: <phenixbb-bounces(a)phenix-online.org> on behalf of "Badmalia, Maulik" <maulik.badmalia(a)uleth.ca> Date: Thursday, February 11, 2021 at 3:37 PM To: "phenixbb(a)phenix-online.org" <phenixbb(a)phenix-online.org> Subject: [phenixbb] unable to run coot hello team phenix, I hope this email finds you well. I recently downloaded phenix for analysing our cryoEM data, before moving to the data analysis I decided to try the tutorial. I decided to use the autosharpen option first, I was able to access the tutorial but when I clicked on coot I repeatedly got a message saying that "Sorry: the 'coot' command could not be located. We have installed Coot to run on a CentOS platform and has the latest version of Coot on the system. I am a novice with linux and phenix. So any help in this regard would be highly appreciated. Do let me know if you have any questions. Regards, Maulik Maulik D. Badmalia Postdoctoral Researcher Laboratory of Medicinal Biophysics Alberta RNA Research and Training Institute Dept. of Chemistry & Biochemistry University of Lethbridge, Lethbridge, AB, Canada

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NIH-funded Postdoc, Membrane Protein Structural Biology (Nebraska, USA)
by Alex Vecchio 11 Feb '21

11 Feb '21

Dear phenixbb Community, I would like to bring your attention to a posting for a 5-year NIH-funded Postdoc position in the Vecchio Lab <https://vecchio-lab.unl.edu> at the University of Nebraska. We are looking for a recent or soon-to-be Ph.D., with experience in membrane protein biochemistry and one structural technique. Our program is established and has capabilities in X-ray crystallography, SAXS, cryo-EM, and now, microED. For more information, please read our ad HERE <https://jobrxiv.org/job/university-of-nebraska-lincoln-27778-membrane-prote…> . To apply or for specific questions, please contact me at avecchio(a)unl.edu. All the best, Alex -- *Alex J. Vecchio, Ph.D.* *Assistant Professor* University of Nebraska–Lincoln Department of Biochemistry Center for Integrated Biomolecular Communication 1901 Vine Street N113 / N114 Beadle Center [Lab / Office] Lincoln, NE 68588 avecchio(a)unl.edu 1 [402] 472-2933

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Call for abstracts (Deadline Feb 18): Michael G. Rossmann Memorial Symposium at the Microscopy & Microanalysis 2021
by Hasan, Syed Saif 11 Feb '21

11 Feb '21

*** MICROSCOPY & MICROANALYSIS 2021 ABSTRACT DEADLINE FEBRUARY 18 *** Dear All, Please note that the deadline for submitting abstract for the Michael Rossmann Memorial Symposium at The Microscopy & Microanalysis conference, held in Pittsburgh, Aug 1-5, 2021 is coming up soon: February 18. So get your abstracts in now! ********************************************************************************************************* We cordially invite you to the “Michael G. Rossmann Memorial Symposium” at the Microscopy & Microanalysis 2021 conference, to be held in Pittsburgh, PA, Aug 1-5, 2021. This symposium focuses on the application of hybrid methods in electron microscopy of viruses, enzymes and other macromolecules, areas in which Michael was a pioneer. The symposium will feature invited talks from Prof. Hao Wu (Harvard Medical School), Prof. Rui Zhao (University of Colorado School of Medicine), and Prof. Chuan (River) Xiao (University of Texas El Paso). We invite abstracts from speakers in academics and industry at all career levels who develop and apply cutting-edge structural biology approaches to the study of viruses, viral proteins, enzymes, membrane proteins, and other systems. Ten abstracts will be selected for 15-minute oral presentations whereas the remaining abstracts will have the opportunity to be selected for poster presentations. Please note that the deadline for abstract submission is February 18, 2021. You will have the opportunity to select your preference for either in-person or virtual presentation. For additional meeting information and to register and submit your abstract, visit: https://www.microscopy.org/MandM/2021/ Deadline for abstract submission: February 18, 2021 We look forward to hosting you and learning about your exciting science at the M&M 2021 meeting. Sincerely, Terje Dokland (University of Alabama at Birmingham, Birmingham AL) S. Saif Hasan (University of Maryland School of Medicine, Baltimore MD)

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phenix.polder how to select atoms by _struct_asym.id
by Agnieszka Kiliszek 11 Feb '21

11 Feb '21

Hi everyone, I would like to generate polder maps in phenix of selected ligand molecules using mmCIF files. For me, the most convenient way of selecting atoms of ligand was to use "_struct_asym.id". Therefore I tried syntax selection segid but I received error "no atoms selected". In pymol similar syntax "segi" works for the same mmCIF file. What can be the reason of the error? Is it a way to select atoms of ligand using "_struct_asym.id" in phenix.polder? Best regards, Agnieszka

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Cryo_fit
by Reza Khayat 10 Feb '21

10 Feb '21

Hi, The gaussian filter used to prepare cryo-EM maps for cryo_fit is equivalent to a low pass filter. Can anyone suggest a resolution to low pass filter the map to? Or is this value empirically determined by screening through a number of resolutions? Thanks. Best wishes, Reza Reza Khayat, PhD Associate Professor City College of New York Department of Chemistry and Biochemistry New York, NY 10031

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Re: [phenixbb] Cryo_fit
by Kim, Doo Nam 09 Feb '21

09 Feb '21

Hi Reza, Can anyone suggest a resolution to low pass filter the map to? Or is this value empirically determined by screening through a number of resolutions? ==> Yes, this is somehow empirically determined. I think that my predecessors of cryo_fit used to use 15 A, 10 A, 5 A resolution cryo-EM maps successively to avoid being trapped in local minima when they fit ribosome atomic structure into these. Relevant references: https://www.phenix-online.org/documentation/faqs/cryo_fit2_FAQ.html#how-can… cascade mdff in https://elifesciences.org/articles/16105 best, Doo Nam From: <phenixbb-bounces(a)phenix-online.org> on behalf of Reza Khayat <rkhayat(a)ccny.cuny.edu> Date: Tuesday, February 9, 2021 at 11:51 AM To: "phenixbb(a)phenix-online.org" <phenixbb(a)phenix-online.org> Subject: [phenixbb] Cryo_fit Check twice before you click! This email originated from outside PNNL. Hi, The gaussian filter used to prepare cryo-EM maps for cryo_fit is equivalent to a low pass filter. Can anyone suggest a resolution to low pass filter the map to? Or is this value empirically determined by screening through a number of resolutions? Thanks. Best wishes, Reza Reza Khayat, PhD Associate Professor City College of New York Department of Chemistry and Biochemistry New York, NY 10031

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Postdoctoral positions at the Massachusetts Institute of Technology
by Thomas Schwartz 07 Feb '21

07 Feb '21

The Schwartz lab <http://schwartzlab.mit.edu/> at Massachusetts Institute of Technology currently has two open positions for postdocs to work on federally funded projects elucidating structure and function of nucleo-cytoplasmic communication. One project involves the structure of the nuclear pore complex (NPC), in particular with the goal to reduce the resolution gap between crystal structures of NPC components and cryo-ET assembly structures. We work at the interface of bio-engineering and structural biology to solve this problem with innovative methods. The second project centers on the mechanical connections between nucleo- and cytoskeleton via LINC complexes. Here, we want to understand the details for LINC complex engagement, with the lamin layer and with actin filaments, in particular. For both projects, the lab has a vast array of reagents, tools, and expertise to build on. MIT has a brand-new, state-of-the-art, fully-staffed cryo-EM facility <https://nanousers.mit.edu/characterizenano-0/automated-cryogenic-electron-m…> equipped with a Talos Arctica / Falcon 3 and a Titan Krios equipped with a Bioquantum/K3 detector and phase plate. We also have cryo-FIB milling capacity. https://nanousers.mit.edu/characterizenano-0/automated-cryogenic-electron-m… <https://nanousers.mit.edu/characterizenano-0/automated-cryogenic-electron-m…> We seek highly motivated individuals with a passion for structural cell biology. A Ph.D. degree in the life sciences with emphasis on biochemistry/structural biology is desired. Candidates should send their application to Thomas Schwartz at tus(a)mit.edu <mailto:[email protected]?subject=postdoc%20position>.

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