I think so. Here's what I have:
export LIBTBX_BUILD=/Users/magnus/Downloads/cctbx_build
export PYTHONPATH=/Users/magnus/Downloads/cctbx_sources:/opt/local/bin:/Users/magnus/Downloads/cctbx_build/lib:/Users/magnus/Downloads/cctbx_sources:/Users/magnus/Downloads/cctbx_sources/boost_adaptbx:/Users/magnus/Downloads/cctbx_sources/libtbx/pythonpath:/usr/local/lib:/opt/local/Library/Frameworks/Python.framework/Versions/2.7/lib/python2.7
export DYLD_LIBRARY_PATH=/Users/magnus/Downloads/cctbx_build/lib